diff --git a/packages/uni-cli-shared/src/json/manifest.ts b/packages/uni-cli-shared/src/json/manifest.ts
index 888b66292e0139b5d6af1600afdff30e044855fe..986aa03a38dd1d7380d275abc7217bd780c8fc33 100644
--- a/packages/uni-cli-shared/src/json/manifest.ts
+++ b/packages/uni-cli-shared/src/json/manifest.ts
@@ -72,3 +72,7 @@ export function getRouterOptions(manifestJson: Record<string, any>): {
 } {
   return extend({}, manifestJson.h5?.router)
 }
+
+export function isEnableTreeShaking(manifestJson: Record<string, any>) {
+  return manifestJson.h5?.optimization?.treeShaking?.enable !== false
+}
diff --git a/packages/uni-cli-shared/src/vite/plugins/inject.ts b/packages/uni-cli-shared/src/vite/plugins/inject.ts
index 746a79b813df611c9a85e688aad03d12e74262c3..a39fb59eefcde1c609414a7a73b3c84a21c31739 100644
--- a/packages/uni-cli-shared/src/vite/plugins/inject.ts
+++ b/packages/uni-cli-shared/src/vite/plugins/inject.ts
@@ -16,7 +16,13 @@ import { walk } from 'estree-walker'
 import { extend } from '@vue/shared'
 import MagicString from 'magic-string'
 
-import { isProperty, isReference, isMemberExpression, isJsFile } from '../utils'
+import {
+  isProperty,
+  isReference,
+  isMemberExpression,
+  isJsFile,
+  isAssignmentExpression,
+} from '../utils'
 
 interface Scope {
   parent: Scope
@@ -46,6 +52,7 @@ export function uniViteInjectPlugin(options: InjectOptions): Plugin {
   delete modules.sourceMap
   delete modules.callback
 
+  const reassignments = new Set<string>()
   const modulesMap = new Map<string, string | [string, string]>()
   const namespaceModulesMap = new Map<string, string | [string, string]>()
   Object.keys(modules).forEach((name) => {
@@ -105,7 +112,12 @@ export function uniViteInjectPlugin(options: InjectOptions): Plugin {
 
       const newImports = new Map()
 
-      function handleReference(node: BaseNode, name: string, keypath: string) {
+      function handleReference(
+        node: BaseNode,
+        name: string,
+        keypath: string,
+        parent?: BaseNode
+      ) {
         let mod = modulesMap.get(keypath)
         if (!mod && hasNamespace) {
           const mods = keypath.split('.')
@@ -128,8 +140,15 @@ export function uniViteInjectPlugin(options: InjectOptions): Plugin {
         if (mod && !imports.has(name) && !scope.contains(name)) {
           if (typeof mod === 'string') mod = [mod, 'default']
           if (mod[0] === id) return false
-
           const hash = `${keypath}:${mod[0]}:${mod[1]}`
+          // 当 API 被覆盖定义后，不再摇树
+          if (reassignments.has(hash)) {
+            return false
+          }
+          if (parent && isAssignmentExpression(parent)) {
+            reassignments.add(hash)
+            return false
+          }
 
           const importLocalName =
             name === keypath ? name : makeLegalIdentifier(`$inject_${keypath}`)
@@ -184,7 +203,7 @@ export function uniViteInjectPlugin(options: InjectOptions): Plugin {
 
           if (isReference(node, parent)) {
             const { name, keypath } = flatten(node)
-            const handled = handleReference(node, name, keypath)
+            const handled = handleReference(node, name, keypath, parent)
             if (handled) {
               this.skip()
             }
diff --git a/packages/uni-cli-shared/src/vite/utils/ast.ts b/packages/uni-cli-shared/src/vite/utils/ast.ts
index b67c4aa275627584903e1fa41626cae465c9bf29..9fb0b2023a576b901901d914e6aefd9754eb74d2 100644
--- a/packages/uni-cli-shared/src/vite/utils/ast.ts
+++ b/packages/uni-cli-shared/src/vite/utils/ast.ts
@@ -4,6 +4,7 @@ import type {
   Property,
   Identifier,
   CallExpression,
+  AssignmentExpression,
   MemberExpression,
   MethodDefinition,
   ExportSpecifier,
@@ -25,6 +26,10 @@ export const isProperty = (node: BaseNode): node is Property =>
 export const isIdentifier = (node: BaseNode): node is Identifier =>
   node.type === 'Identifier'
 
+export const isAssignmentExpression = (
+  node: BaseNode
+): node is AssignmentExpression => node.type === 'AssignmentExpression'
+
 export const isCallExpression = (node: BaseNode): node is CallExpression =>
   node.type === 'CallExpression'
 
diff --git a/packages/uni-h5-vite/src/plugins/inject.ts b/packages/uni-h5-vite/src/plugins/inject.ts
index 1e9bc983ede72e078087771b71985f6be8bb5b68..952b63efa9ea63aee6a9083c21b78708530a520f 100644
--- a/packages/uni-h5-vite/src/plugins/inject.ts
+++ b/packages/uni-h5-vite/src/plugins/inject.ts
@@ -10,6 +10,8 @@ import {
   buildInCssSet,
   uniViteInjectPlugin,
   isCombineBuiltInCss,
+  isEnableTreeShaking,
+  parseManifestJsonOnce,
 } from '@dcloudio/uni-cli-shared'
 
 const apiJson = require(path.resolve(__dirname, '../../lib/api.json'))
@@ -49,17 +51,27 @@ export function uniInjectPlugin(): Plugin {
       }
     })
   }
-  const injectPlugin = uniViteInjectPlugin(
-    extend(uniInjectPluginOptions, {
-      callback,
-    })
-  )
+  let injectPlugin: Plugin
+
   return {
     name: 'vite:uni-h5-inject',
     apply: 'build',
     enforce: 'post',
     configResolved(config) {
       resolvedConfig = config
+      const enableTreeShaking = isEnableTreeShaking(
+        parseManifestJsonOnce(process.env.UNI_INPUT_DIR)
+      )
+      if (!enableTreeShaking) {
+        // 不启用摇树优化，移除 wx、uni 等 API 配置
+        delete uniInjectPluginOptions['wx.']
+        delete uniInjectPluginOptions['uni.']
+      }
+      injectPlugin = uniViteInjectPlugin(
+        extend(uniInjectPluginOptions, {
+          callback,
+        })
+      )
     },
     transform(code, id) {
       return injectPlugin.transform!.call(this, code, id)
diff --git a/packages/uni-h5-vite/src/plugins/pagesJson.ts b/packages/uni-h5-vite/src/plugins/pagesJson.ts
index 9d4b1b0fe6eeba5fff80d69adf039e29ec9e4d07..06d91be2aa0b10ab7719d9fdbc6beed1cb02d80b 100644
--- a/packages/uni-h5-vite/src/plugins/pagesJson.ts
+++ b/packages/uni-h5-vite/src/plugins/pagesJson.ts
@@ -11,6 +11,8 @@ import {
   normalizePagesRoute,
   normalizePagePath,
   normalizePath,
+  isEnableTreeShaking,
+  parseManifestJsonOnce,
 } from '@dcloudio/uni-cli-shared'
 
 export function uniPagesJsonPlugin(): Plugin {
@@ -79,8 +81,11 @@ function getGlobal(ssr?: boolean) {
 // 兼容 wx 对象
 function registerGlobalCode(config: ResolvedConfig, ssr?: boolean) {
   const name = getGlobal(ssr)
-  if (config.command === 'build' && !ssr) {
-    // 非SSR的发行模式，补充全局 uni 对象
+  const enableTreeShaking = isEnableTreeShaking(
+    parseManifestJsonOnce(process.env.UNI_INPUT_DIR)
+  )
+  if (enableTreeShaking && config.command === 'build' && !ssr) {
+    // 非 SSR 的发行模式，补充全局 uni 对象
     return `${name}.uni = {};${name}.wx = {}`
   }