diff --git a/python/paddle/fluid/dygraph/layers.py b/python/paddle/fluid/dygraph/layers.py
index 6fa531c573daa7bdc6b2c043ae29e8363c7bcaa8..10786c662072cce6fbea642464c4aff3de552ae1 100644
--- a/python/paddle/fluid/dygraph/layers.py
+++ b/python/paddle/fluid/dygraph/layers.py
@@ -1023,13 +1023,20 @@ class Layer(core.Layer):
                         self._non_persistable_buffer_names_set.add(name)
                     _buffers[name] = value
                 elif _buffers is not None and name in _buffers:
-                    if value is not None:
+                    # Note(Aurelius84): In Dy2stat, the value of the Buffer may be modified in 
+                    # decorated function, such as `self.buffer = new_tensor`. So we update its
+                    # value via `assign`.
+                    if type(value) == framework.Variable:
+                        from paddle import assign
+                        assign(value, _buffers[name])
+                    elif value is not None:
                         raise TypeError(
                             "assignment to buffers '{}' should be of type core.VarBase or None, but got '{}'"
                             .format(name, type(value).__name__))
-                    # Assigning None will remove the buffer, but if re-assign a new varBase to it,
-                    # it will be remarked as a buffer with same `persistable` attribute.
-                    _buffers[name] = None
+                    else:
+                        # Assigning None will remove the buffer, but if re-assign a new varBase to it,
+                        # it will be remarked as a buffer with same `persistable` attribute.
+                        _buffers[name] = None
                 else:
                     object.__setattr__(self, name, value)
 
diff --git a/python/paddle/fluid/tests/unittests/test_base_layer.py b/python/paddle/fluid/tests/unittests/test_base_layer.py
index 875f6211a7fbd98463d98dff91d93cc1b431fc86..31879dae0dad06d75a1ad5c6b6780ef2c3d2b93b 100644
--- a/python/paddle/fluid/tests/unittests/test_base_layer.py
+++ b/python/paddle/fluid/tests/unittests/test_base_layer.py
@@ -15,9 +15,11 @@
 import unittest
 import numpy as np
 
+import paddle
 import paddle.fluid as fluid
 from paddle.fluid.dygraph import to_variable
 from paddle.fluid.framework import ParamBase
+from paddle.jit import ProgramTranslator
 
 
 class L1(fluid.Layer):
@@ -288,5 +290,46 @@ class TestBuffer(unittest.TestCase):
         self.assertTrue(np.array_equal(var1.numpy(), var2.numpy()))
 
 
+class BufferNetWithModification(paddle.nn.Layer):
+    def __init__(self, shape):
+        super(BufferNetWithModification, self).__init__()
+
+        self.buffer1 = paddle.zeros(shape, 'int32')
+        self.buffer2 = paddle.zeros(shape, 'int32')
+
+    @paddle.jit.to_static
+    def forward(self, x):
+        self.buffer1 += x
+        self.buffer2 = self.buffer1 + x
+
+        out = self.buffer1 + self.buffer2
+
+        return out
+
+
+class TestModifiedBuffer(unittest.TestCase):
+    def setUp(self):
+        paddle.disable_static()
+        self.prog_trans = ProgramTranslator()
+        self.shape = [10, 16]
+
+    def _run(self, to_static=False):
+        self.prog_trans.enable(to_static)
+
+        x = paddle.ones([1], 'int32')
+        net = BufferNetWithModification(self.shape)
+        out = net(x)
+
+        return out, net.buffer1, net.buffer2
+
+    def test_modified(self):
+        dy_outs = self._run(False)
+        st_outs = self._run(True)
+
+        for i in range(len(dy_outs)):
+            self.assertTrue(
+                np.array_equal(dy_outs[i].numpy(), st_outs[i].numpy()))
+
+
 if __name__ == '__main__':
     unittest.main()