diff --git a/changelogs/unreleased/unicorn-sampler-fix.yml b/changelogs/unreleased/unicorn-sampler-fix.yml
new file mode 100644
index 0000000000000000000000000000000000000000..3f0e509f15f1636d12810ff67b8042c9e024a0c2
--- /dev/null
+++ b/changelogs/unreleased/unicorn-sampler-fix.yml
@@ -0,0 +1,5 @@
+---
+title: Make sure UnicornSampler is started only in master process.
+merge_request: 30215
+author:
+type: fixed
diff --git a/config/initializers/7_prometheus_metrics.rb b/config/initializers/7_prometheus_metrics.rb
index 54cdefc2a10c48ade75f4fe08b7955d675509979..5db72141fcd1e685ac937587b43fc2ee6d294bbd 100644
--- a/config/initializers/7_prometheus_metrics.rb
+++ b/config/initializers/7_prometheus_metrics.rb
@@ -29,15 +29,13 @@ if !Rails.env.test? && Gitlab::Metrics.prometheus_metrics_enabled?
   Gitlab::Cluster::LifecycleEvents.on_worker_start do
     defined?(::Prometheus::Client.reinitialize_on_pid_change) && Prometheus::Client.reinitialize_on_pid_change
 
-    if defined?(::Unicorn)
-      Gitlab::Metrics::Samplers::UnicornSampler.initialize_instance(Settings.monitoring.unicorn_sampler_interval).start
-    end
-
     Gitlab::Metrics::Samplers::RubySampler.initialize_instance(Settings.monitoring.ruby_sampler_interval).start
   end
 
-  if defined?(::Puma)
-    Gitlab::Cluster::LifecycleEvents.on_master_start do
+  Gitlab::Cluster::LifecycleEvents.on_master_start do
+    if defined?(::Unicorn)
+      Gitlab::Metrics::Samplers::UnicornSampler.initialize_instance(Settings.monitoring.unicorn_sampler_interval).start
+    elsif defined?(::Puma)
       Gitlab::Metrics::Samplers::PumaSampler.initialize_instance(Settings.monitoring.puma_sampler_interval).start
     end
   end